Manzoor A. Wani - Research Associate II B/H
wani.4@osu.edu

• Education
• Academic
• Research and Work Experience
• Memberships in Professional Organizations
• Publications
• Academic Presentations and Research
• Conferences Attended
• Personal Strengths
• References

Education

Academic

Obtained highest (first) division for my master’s work. Strong theoretical background in course taught in biochemistry. Hands on practical training in diverse laboratory techniques.

Course: physical and organic chemistry; carbohydrates, lipids, vitamins. hormones; proteins and enzymes; cell biology and clinical biochemistry; metabolism and biological oxidation; molecular biology and immunology; biochemical techniques, biotechnology and microbiology.

Laboratory: Quantitative analysis of carbohydrates; various assay procedures for amino acids and proteins; colorimetry of various biochemicals of clinical relevance; enzymology related experiments including subcellular fractionation, isolation and estimation of phosphatases; Gel filtration, column and ion-exchange chromatography, native and SDS-PAGE, Western Blot; Microbiology experiments including media preparations, sterile techniques, bacterial culture, staining isolation and identification; Serum protein analysis; immobilization of enzymes and RBCs; Immunological assays including ELISA based on phosphatase colorimetry; purification and quantification of RNA and DNA from animal sources, hybridization and Tm analysis, Northern and Southern Blot Analysis.

Research and Work Experience

Skills: DNA and RNA purification from bacterial and mammalian cells; generating specific gene fragments by PCR and cloning in specific vector sequences; subcloning; restriction endonuclease analysis; transformation of bacteria and transfection of mammalian cells; 32P-end-labelling DNA to prepare probe and hybridization with oligo probes; experience in handling radioisotopic material and hazardous chemicals; Radio-iodination of proteins using I-125 and I-131; dual-isotope labeling; injecting and analyzing radio-iodinated proteins into animals; DNA sequencing; recombinant DNA techniques, spectrophotometric and fluorometric analysis of DNA; PCR and RT-PCR, Northern, Southern and Western blotting; autoradiography; immuno-precipitation; phosphorimager analysis; heteroduplex analysis; ligation-mediated PCR; analysis of gene, strand and site-specific repair; developing polyclonal antibodies in rabbits for DNA repair immunoassays; cellular apoptosis related assays; measurement of cell survival and cytotoxicity assays; reporter assays; expression and purification of proteins; affinity and ion-exchange chromatography; FPLC; extensive experience with both suspension and adherent mammalian cell culture and culturing primary cells and animal handling; flow cytometry; analyzes data for reports, publications and presentations; assists in writing manuscripts, abstracts, grants and supervises students; lab supervisory experience; excellent proficiency in work related computer skills and DNA sequence alignment softwares.

Memberships in Professional Organizations

American Association for the Advancement of Science
American Association for Cancer Research

Publications

Wani, Manzoor A., and Clark L. Anderson. (2005) A novel beta2-microglobulin gene mutation: cause of an unknown familial syndrome. (Manuscript in Preparation)

Wani, Manzoor A., and Wani, Altaf.A. Dynamics of Nucleotide Excision Repair in Chromatin. (2005). Review (Submitted: Environmental and Molecular Mutagenesis).

Qianzheng Zhu, Manzoor A. Wani, Jihong Yao, and Altaf A. Wani. (2005) The Ubiquitin-mediated proteasome pathway is involved in transcriptional activation. (Submitted: Nucleic Acid Research).

Wani, M.A., Qi-en Wang, Zhu, Q.Z., Chen, J and Wani, A.A. (2005) Cellular ubiquitination and proteasomal functions positively modulate mammalian nucleotide excision repair. Molecular Carcinogenesis 42(1):53-64.

Wang, Qi-en., Zhu, Qianzheng, Wani, Manzoor A., Chen, Jianming, and Wani, Altaf A. (2003). Tumor Suppressor p53 Dependent Recruitment of Nucleotide Excision Repair Factors XPC and TFIIH to DNA Damage. DNA Repair 2(5):483-99.

Wani, Manzoor A., El-Mahdy, M., Hamada, F., Zhu, Qianzheng, Wang, Qien, and Wani, Altaf A.(2002). Efficient Repair of bulky anti-BPDE-DNA adducts from non-transcribed DNA strand requires functional p53 but not p21waf1/cip1 and Rb. Mutation Research 505(1-2):13-25.

Wani, Manzoor.A., Yao, Jihong, Zhu, Qianzheng, and Wani, Altaf A.(2002) Human cells deficient in p53 regulated p21waf1/cip1 expression exhibit normal nucleotide excision repair of UV-induced DNA damage. Carcinogenesis 23(3):101-108.

Zhu, Qianzheng, Yao, Jihong, Wani, Manzoor, and Wani, Altaf A. (2001) Mdm2 mutant defective in binding p300 promotes ubiquitination but not degradation of p53: Evidence for the role of p300 in integrating ubiquitination and proteolysis. J. Biol. Chem 276(32):29695-29701.

Oianzheng Zhu, Manzoor A. Wani, Altaf A. Wani. (2001) Human Homologue of Yeast Rad23 protein A Interacts with p300/Cyclic AMP-responsive Element Binding (CREB)-binding Protein to Down-Regulate Transcriptional Activity of p53. Advances in Brief. Cancer Research 61(1):64-70.

Wani, M.A., Zhu, Q.Z., El-Mahdy, M., Venkatachalam, S., and Wani, A.A. (2000) Enhanced sensitivity to Anti-BPDE DNA damage correlates with Decreased Global Genomic repair due to Abrogated p53 function in human cells. Cancer Research 60(8):2273-2280.

Qianzheng Zhu, Manzoor A. Wani, Mohammad El-Mahdy, and Altaf A. Wani. (2000) Decreased DNA Repair Efficiency by Loss of p53 Function Preferentially Affects Removal of cyclobutane Pyrimidine Dimers from Non-transcribed Strand and Slow Repair Sites in Transcribed Strand. J. Biol. Chem 275(15):11492-11497.

El-Mahdy, M., Hamada, F., Wani, M.A., and Wani, A.A. (2000) p53-degradation by HPV-16E6 preferentially affects the removal of cyclobutane pyrimidine dimers from non-transcribed strand and sensitizes mammary epithelial cells to UV-irradiation. Mutation Research 459(2):135-145.

Qianzheng Zhu, Manzoor A. Wani, Mohammad El-Mahdy, and Altaf A. Wani. (2000) Modulation of transcriptional activity of p53 by ultraviolet radiation implicates linkage between p53 pathway and DNA repair by damage recognition. Molecular Carcinogenesis 28(4):215-224.

Wani, M.A., Zhu, Q.Z., El-Mahdy, M. and Wani, A.A. (1999) Influence of p53 tumor suppressor protein on bias of DNA repair and apoptotic response in human cells. Carcinogenesis 20(5):765-772.

Academic Presentations and Research Conferences Attended

Attended 12th International Congress of Immunology and 4th Annual Conference of FOCIS, July 18-23, 2004, Montreal, Canada.

Attended 4th Annual Midwest DNA Repair Symposium " DNA Double Strand Break Repair" May 4-5, 2002, University of Cincinnati, Ohio.

Attended Ninety Third Annual Meeting, American Association for Cancer Research, April 6-10, 2002, San Francisco.

Attended Fourth Annual Comprehensive Cancer Center Scientific Meeting, Genomics and Cancer Biology, Jan 11 2002, Dublin, Columbus, Ohio.

Attended Ninety Second Annual Meeting, American Association for Cancer Research, March 24-28, 2001, New Oreleans.

Attended Midwest Regional Conference on “Pharmacology of DNA Topoisomerases and DNA Repair” Franz Theodore Stone Lab, Put-in-Bay, Ohio, May 21-23, 1999.

Attended Annual Ohio Cancer Symposium, Sept 17-18, 1998, The Ohio State University, Ohio.

Attended Eighty Ninth Annual Meeting, American Association for Cancer Research, March 28th-April 1st, 1998, New Oreleans.

Attended International Conference on Eukaryotic Expression Vector Systems, National Institute of Immunology, New Delhi, Jan. 4-8, 1996.

Attended 5th Annual Conference of Indian Academy of Vaccinology and Immunobiology, ICGEB, Dec 14, 1995.

Attended 3rd South Asian Human Genome Conference, ICGEB,New Delhi, Dec 3-7, 1995.

Wani, M.A., S. Mohanty, and C.L. Anderson. Genotype of two patients manifesting the phenotype of an FcRn-deficient mouse. Clinical and Investigative Medicine 27(4) August 2004.

Mohanty, S., M.A. Wani, and C.L. Anderson. Widespread expression of the MHC-related receptor for IgG (FcRn).Clinical and Investigative Medicine 27(4) August 2004.

Wani, M.A., Zhu, Q.Z., Qi-en Wang., Chen, J and Wani, A.A. Inactivation of Ubiquitin-activating enzyme (E1) causes a decrease in both GGR and TCR of UV-induced DNA damage in mouse cells. Proceedings, Ninety Fourth Annual Meeting, American Association for Cancer Research, April 5-9, 2003, Toronto, Canada.

Qi-en Wang, Zhu,Q.Z., Wani, M.A., Chen, J., and Wani, A.A. The Subuclear Distribution of NER Factors and Their Response to UV Irradiation. Proceedings, Ninety Fourth Annual Meeting, American Association for Cancer Research, April 5-9, 2003, Toronto, Canada.

Zhu, Q.Z., Qi-en Wang., Chen, J., Wani, M.A and Wani, A.A. Influences of Ubiquitin on Transcription and Degradation Revealed by Linear Fusion of Ubiquitin Moiety to Transcriptional Activators. Proceedings, Ninety Fourth Annual Meeting, American Association for Cancer Research, April 5-9, 2003, Toronto, Canada.

Wani, M.A., El-Mahdy, M., Hamada, F.M., Zhu, Q.Z., Qien Wang., and Wani, A.A. Repair of bulky anti-BPDE DNA adducts from the non-transcribed DNA strand requires functional p53 independent of p21 waf1/cip1 expression. 18th UICC International Cancer Congress, June 30- July 5, 2002, Oslo-Norway.

Qianzheng Zhu., Jihong Yao., Manzoor A. Wani., Qien Wang., and Altaf A. Wani. Functional interaction between gene transactivation, ubiquitylation and proteasome-mediated protein degradation. 18th UICC International Cancer Congress, June 30- July 5, 2002, Oslo-Norway.

Wani, Manzoor.A., El-Mahdy, M., Hamada, F., Zhu, Qianzheng., Wang, Qien., and Wani, Altaf.A. Efficient Repair of bulky anti-BPDE-DNA adducts from non-transcribed DNA strand requires functional p53 but not p21waf1/cip1 and Rb. 4th Annual Midwest DNA Repair Symposium " DNA Double Strand Break Repair" May 4-5, 2002, University of Cincinnati, Ohio.

Qianzheng Zhu., Manzoor A. Wani., Jihong Yao., and Altaf A. Wani. The Ubiquitin-mediated proteasome pathway is required for efficient transcriptional activation. 4th Annual Midwest DNA Repair Symposium " DNA Double Strand Break Repair" May 4-5, 2002, University of Cincinnati, Ohio.

Wang, Qi-en., Zhu, Qianzheng., Wani, Manzoor.A., Chen, Jianming., and Wani, Altaf.A. Effect of p53 function on the in situ recruitment of nucleotide excision repair components. 4th Annual Midwest DNA Repair Symposium " DNA Double Strand Break Repair" May 4-5, 2002, University of Cincinnati, Ohio.

Qianzheng Zhu., Jihong Yao., Manzoor A. Wani., Qien Wang., and Altaf A. Wani. Functional interaction between gene transactivation, ubiquitylation and proteasome-mediated protein degradation. Proceedings, Ninety Third Annual Meeting, American Association for Cancer Research, April 24-28, 2002, San Francisco.

Wani, Manzoor.A., Yao, Jihong., Zhu, Qianzheng., and Wani, Altaf.A. Disrupting p53-dependent transcription does not affect global genomic DNA repair: involvement of p53 and p21waf1 in different sub-pathway of DNA repair. Proceedings, Ninety second Annual Meeting, American Association for Cancer Research, March 24-28, 2001, New Orleans.

Zhu, Qianzheng., Yao, Jihong., Wani, Manzoor., and Wani, Altaf.A. Ubiquitin proteolytic pathway is involved in both p53 transactivation and degradation. Proceedings, Ninety second Annual Meeting, American Association for Cancer Research, March 24-28, 2001, New Orleans.

Wani, M.A., Zhu, Q.Z., El-Mahdy, M., and Wani, A.A. Influence of p53 tumor suppressor protein on bias of DNA repair and apoptotic response in human cells. Midwest Regional Conference, May 22nd, 1999, Cleveland, Ohio.

Zhu, Q.Z., Wani, M.A., and Wani, A.A. Loss of functional p53 in Li-Fraumeni Syndrome fibroblasts preferentially affects DNA repair of non-transcribed strands but not transcribed strands. Midwest Regional Conference, May 22nd, 1999, Cleveland, Ohio.

El-Mahdy, M., Hamada, F., Zhu, Q.Z., Wani, M.A., and Wani, A.A. p53-degradation by HPV 16E6 affects global DNA reapir but not trancription-coupled repair and sensitizes human mammary epithelial cells to DNA damage. Midwest Regional Conference, May 22nd, 1999, Cleveland, Ohio.

Wani, M.A., Zhu, Q.Z., El-Mahdy, M., and Wani, A.A. p53 independent induction of cell cycle regulatory proteins following DNA damage in Human cells. Annual Ohio Cancer Symposium, Sept17-18, 1998, Ohio.

El-Mahdy, M., Hamada, F., Wani, M.A., and Wani, A.A. Ubiquitin-mediated protein degradation sensitizes human mammary epithelial cells to uv-irradiation. Annual Ohio Cancer Symposium, Sept17-18, 1998, Ohio.

Wani, M.A., Venkatachalam, S., Zhu, Q.Z., El-Mahdy. and Wani, A.A. p53 independent induction of cell cycle regulatory proteins following DNA damage in human cells. Proceedings, Vol. 39, 1998, Eighty Ninth Annual Meeting, American Association for Cancer Research, April 1st, 1998, New Orleans.

Zhu, Q.Z., Wani, M.A., Swaidani, S and Wani, A.A, Functional p53 protein is required for the DNA damage repair of non-transcribed strand but not transcribed strand.. Proceedings, Vol. 39, 1998, Eighty Ninth Annual Meeting, American Association for Cancer Research, April 1st, 1998, New Orleans.

El-Mahdy, M., Hamada, F., Wani, M.A., and Wani, A.A. Ubiquitin-mediated protein degradation sensitizes human mammary epithelial cells to UV-irradiation. Proceedings, Vol. 39, 1998, Eighty Ninth Annual Meeting, American Association for Cancer Research, April 1st, 1998, New Orleans.

Personal Strengths

Exceptional ability to conduct independent experiments, trouble shoot assays and take instructions to meet project goals; extensive experience in various aspects of research in biochemistry and molecular biology; work with other scientists with ease and get along with anybody; highly motivated, thoughtful, responsible, athletic, and productive.

References

Available upon request.