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Chaity Chaudhury, Ph.D. - Post-Doctoral Researcher
chaudhury.3@osu.edu
OBJECTIVE
Post-doctoral
training in cancer signaling, immunology and therapy
which will help enhance my scientific skills and creativity
towards establishing a successful career in research.
EDUCATION
SPECIFIC
SKILLS/TECHNIQUES
Molecular and Cell Biology
DNA isolation, PCR, cloning, site directed mutagenesis; generating
transient and stable transfectants in mammalian cell systems,
reporter assays; flow cytometry
Immunology
ELISA; Western blot analysis; immunoprecipitation; phagocytosis
assay
Animal Handling
Experienced in small animal handling; intravenous and intraperitoneal
injections; sampling blood through the retro-orbital plexus and
inferior venaecava; generation and pharmacokinetic data analysis
of radio-labeled protein distribution and determination of protein
half lives from decay profiles in animal models
Protein Chemistry
Optimizing protein expression in both bacterial and mammalian
expression systems; purification by size exclusion, affinity and
ion exchange chromatography using FPLC; radiolabeling proteins;
characterization of protein-protein interaction using Surface
Plasmon Resonance (BIACORE); thermodynamic characterization of
protein-protein interaction by Isothermal Titration Calorimetry
(VP ITC Microcal)
Enzymology
Steady state enzyme kinetics; purification of small molecule inhibitors
of peptide deformylase using HPLC and characterization of the
inhibition kinetics to identify the high potency inhibitors
Data analysis and data mining
Direct working experience with Vector NTI; RASMOL; Quantity One;
Kaleidagraph; Surface Plasmon Resonance data analysis softwares
BIAevaluation, CLAMP and scrubber, Certified in kinetic and affinity
analysis course conducted by BIAcore; Isothermal Titration Calorimetry
data analysis using Origin 7; MS Office; Reference Manager.
RESEARCH
EXPERIENCE
• Discovered a novel receptor for binding, transporting
and enhancing the lifespan of albumin.
• Showed that the half-life of albumin is shortened and
the serum steady state concentration is low in FcRn-deficient
mice.
• Purified recombinant FcRn from a stably transfected mammalian
expression system, and characterized the kinetics (on and off
rate, affinity) of interaction using Surface Plasmon Resonance.
• Characterized the stoichiometry and energetics of the
FcRn-albumin interaction using isothermal titration calorimetry.
• Identified the conserved histidine residues that may be
involved in mediating the FcRn-albumin binding. Work is underway
to create histidine mutants of FcRn and albumin to identify the
residues involved in the interaction.
• Performed preliminary studies to assess the role of FcRn
in mediating transendothelial transport of albumin.
• Purified mutants of SHIP, syk, PI-3 kinase and optimized
the protocol for transfection of monocytic cell lines.
• Purified inhibitors of the enzyme peptide deformylase
created from a combinatorial library by HPLC and characterized
the potency of the inhibitors by determining Km and Kcat.
• Showed that deficiency of HFE hastens degradation of transferrin
in mice.
Summer research projects:
• Industrial research project titled "Optimizing anaerobic
biodegradation of xenobiotic industrial effluents" at National
Environmental Engineering Research Institute, India, 1997.
• Summer Research trainee, Center for Biotechnology, Jawaharlal
Nehru University, New Delhi, India, 1996.
TEACHING
EXPERIENCE
| 1999-2000 |
Graduate
Teaching Associate, Department of Chemistry, Ohio
State University. Responsibilities included
teaching and laboratory supervision of General chemistry
and Organic chemistry courses for undergraduate
science students; grading exams, quizzes and laboratory
reports. |
| 1998-1999 |
Graduate
Teaching Associate, Department of Biology, Ohio
State University. Responsibilities included
laboratory supervision of General Biology courses
for undergraduate science students and grading laboratory
reports. |
SUPERVISORY
EXPERIENCE
Supervised
research associates and undergraduate work study students in preparing
buffers, perform assays, protein expression and purification techniques,
tissue culture techniques.
HONORS AND ACTIVITIES
| 1996-1998 |
Sunderlal
Sahu Gold medal and Kaple gold medal by Nagpur University,
India for securing the first position in M.S. Biochemistry |
| 1996-1997 |
Umesh
Khante memorial award, for securing first position
in M.S.-I for the academic year by the Department
of Biochemistry, Nagpur University, India |
| 1996-1998 |
Graduate
school representative of the Dept of Biochemistry,
Nagpur University to the University Counci |
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National
Scholarship for graduate education, awarded by the
Ministry of Human Resource Development, Government
of India. |
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Ranked
first all three years of B.S.. |
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Honored
merit award, Nagpur University for excellent performance
in B.S.. |
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Best
student award, St. Francis De Sales College for
excellent academic performance during undergraduate
work. |
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Late.
Smt. Kamla Laxmi Nautamlal Trivedi prize, for securing
highest marks in Botany in B.S.. |
PUBLICATIONS
Chaudhury C, Brooks CL, Curry S, Robinson JM, Anderson CL. (2005)
FcRn-albumin binding is mediated via histidine residues. (In preparation)
Chaudhury
C, Kim J, Wani MA, Hayton WL, Robinson JM, Anderson CL. HFE increases
transferrin half-life. (To be submitted) (2005)
Chaudhury C, Brooks CL, Carter DC, Robinson JM, Anderson CL. Albumin
binding by the MHC-related Fc receptor (FcRn): Albumin and IgG
are salvaged independently by similar mechanisms. (submitted EMBO
Journal) (2005)
Bronson
CL, Kim J, Chaudhury C, Hayton WL, Oberyszyn TM, Roopenian DC,
Anderson CL. FcRn-mediated transendothelial flux of albumin and
IgG. (to be submitted) (2005)
Chaudhury
C, Mehnaz S, Robinson JM, Hayton WL, Pearl DK, Roopenian DC, Anderson
CL. The major histocompatibility complex-related Fc receptor for
IgG (FcRn) binds albumin and prolongs its lifespan. J Exp
Med. 2003 Feb 3;197(3):315-22.
Wei
Y, Yi T, Huntington KM, Chaudhury C, Pei D. Identification of
a potent peptide deformylase inhibitor from a rationally designed
combinatorial library. J Comb Chem. 2000 Nov-Dec;2(6):650-7.
Beebe
KD, Shin J, Peng J, Chaudhury C, Khera J, Pei D. Substrate recognition
through a PDZ domain in tail-specific protease. Biochemistry.
2000 Mar 21;39(11):3149-55.
SEMINAR
AND SYMPOSIA PRESENTATIONS
Poster
titled: Biochemical characterization of the FcRn:Albumin interaction
12th International Congress of Immunology, 2004, Montreal, Canada.
Poster titled: The MHC-related Fc receptor for IgG (FcRn) contributes
to the transendothelial flux of albumin. 12th International Congress
of Immunology, 2004, Montreal, Canada.
Poster titled: Biochemical characterization of the FcRn:Albumin
interaction. OSBP Departmental poster session, OSU, May 2004.
Poster titled: FcRn: novel protection receptor for albumin. August
2002 FASEB Immuno-receptor international conference, Tuscon, AZ.
Poster titled: FcRn: novel protection receptor for albumin. OSBP
Departmental poster session, OSU, May 2002.
National Symposium on Disease Diagnostics organized by National
Environmental Engineering Research Institute, India 1998.
International Seminar on Current Trends in Biochemical research
and Education, organized by the Department of Biochemistry, Nagpur
University, India 1997.
REFERENCES
Clark
L. Anderson, M.D.
Professor, Department of Internal of Medicine
The Ohio State University
Room 415 Heart & Lung Research Institute (DHLRI)
473 West Twelfth Avenue
Columbus, OH 43210-1228
Phone: (614) 247-7650
FAX: (614) 247-7669
anderson.48@osu.edu
Charles
L. Brooks, Ph.D.
Professor, Department of Veterinary Biosciences
The Ohio State University
1925 Coffey Road
Columbus, OH 43210-1093
Phone: (614) 292-9641
FAX: (614) 292-6473
brooks.8@osu.edu
Chandan
K. Sen, Ph.D.
Professor, Surgery and Molecular & Cellular Biochemistry
The Ohio State University
473 West Twelfth Avenue
Columbus, OH 43210-1228
Phone: (614) 247-7658
FAX: (614) 247-7818
sen-1@medctr.osu.edu
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